Peroxidase Catalyzed Decoloration of Orange II Coupled with Glucose-Glucose Oxidase System

Direct Automated Determination of Glucose by a Glucose Oxidase-peroxidase System.

THE DETERMINATION of glucose by the glucose oxidase-peroxidase system has been simplified by Hill and Kessler (2) with the use of an automated system first described by Skeggs (1). Wincy and Marks (3) have also developed a method using o-tolidine in the automated system. These procedures require a large quantity of sample and use of a dialyzer to separate the protein before color development. T...

Measurement of glucose in plasma, with use of immobilized glucose oxidase and peroxidase.

We describe a novel fluorometric technique for simple, rapid, and economical assay of glucose by use of immobilized glucose oxidase and peroxidase. A cylindrical magnetic stirrer was specially designed to hold the immobilized enzymes firmly and to allow the reaction minzymic transformation quickly. Blood plasma can be assayed directly with no pretreatment. Ascrobic acid, uric acid, and creatini...

Determination of blood glucose using an oxidase-peroxidase system with a non-carcinogenic chromogen.

Manual and automated methods for the determination of blood glucose have been devised using an oxidase/peroxidase system, with dl adrenaline, a non-carcinogen, as oxygen acceptor. The manual technique employs a stable single solution protein precipitant and the other reagents used are also stable. The automated methods are operated at 40/hr sample speed and washover between samples, over a very...

Use of glucose oxidase, peroxidase, and O-dianisidine in determination of blood and urinary glucose.

Factors affecting accuracy and time requirements of a glucose oxidase-peroxidase assay for determination of glucose.

Accurate and rapid assays for glucose are desirable for analysis of glucose and starch in food and feedstuffs. An established colorimetric glucose oxidase-peroxidase method for glucose was modified to reduce analysis time and evaluated for factors that affected accuracy. Time required to perform the assay was reduced by approximately 40% by decreasing incubation time and removing steps that do ...